BAF-1 mobility is regulated by environmental stresses
نویسندگان
چکیده
Barrier to autointegration factor (BAF) is an essential component of the nuclear lamina that binds lamins, LEM-domain proteins, histones, and DNA. Under normal conditions, BAF protein is highly mobile when assayed by fluorescence recovery after photobleaching and fluorescence loss in photobleaching. We report that Caenorhabditis elegans BAF-1 mobility is regulated by caloric restriction, food deprivation, and heat shock. This was not a general response of chromatin-associated proteins, as food deprivation did not affect the mobility of heterochromatin protein HPL-1 or HPL-2. Heat shock also increased the level of BAF-1 Ser-4 phosphorylation. By using missense mutations that affect BAF-1 binding to different partners we find that, overall, the ability of BAF-1 mutants to be immobilized by heat shock in intestinal cells correlated with normal or increased affinity for emerin in vitro. These results show BAF-1 localization and mobility at the nuclear lamina are regulated by stress and unexpectedly reveal BAF-1 immobilization as a specific response to caloric restriction in C. elegans intestinal cells.
منابع مشابه
Barrier to Autointegration Factor Becomes Dephosphorylated during HSV-1 Infection and Can Act as a Host Defense by Impairing Viral DNA Replication and Gene Expression
BAF (Barrier to Autointegration Factor) is a highly conserved DNA binding protein that senses poxviral DNA in the cytoplasm and tightly binds to the viral genome to interfere with DNA replication and transcription. To counteract BAF, a poxviral-encoded protein kinase phosphorylates BAF, which renders BAF unable to bind DNA and allows efficient viral replication to occur. Herein, we examined how...
متن کاملCoordination of Kinase and Phosphatase Activities by Lem4 Enables Nuclear Envelope Reassembly during Mitosis
Mitosis in metazoa requires nuclear envelope (NE) disassembly and reassembly. NE disassembly is driven by multiple phosphorylation events. Mitotic phosphorylation of the protein BAF reduces its affinity for chromatin and the LEM family of inner nuclear membrane proteins; loss of this BAF-mediated chromatin-NE link contributes to NE disassembly. BAF must reassociate with chromatin and LEM protei...
متن کاملShort communication Effect of ba®lomycin A1 on the growth of Japanese encephalitis virus in Vero cells
We studied the effect of ba®lomycin A1 (Baf-A1), a novel and highly speci®c inhibitor for vacuolar-type proton (V-H) pump, on the growth of Japanese Encephalitis virus (JEV) in Vero cells. Viral ̄uorescence microscopic study showed that Baf-A1 induced the complete disappearance of acidi®ed compartments such as endosomes and lysosomes in Vero cells by the treatment with 0.1 mM Baf-A1 for 1 h at 3...
متن کاملDepletion of the protein kinase VRK1 disrupts nuclear envelope morphology and leads to BAF retention on mitotic chromosomes
Barrier to autointegration factor (BAF), which is encoded by the BANF1 gene, binds with high-affinity to double-stranded DNA and LEM domain-containing proteins at the nuclear periphery. A BANF1 mutation has recently been associated with a novel human progeria syndrome, and cells from these patients have aberrant nuclear envelopes. The interactions of BAF with its DNA- and protein-binding partne...
متن کاملChromosomes leave envelope for karyosome
I n the large volume of an oocyte, chromosomes huddle together before the meiotic spindle forms. Fashioning this huddled mass—called a karyosome—requires that chromosomes fi rst be released from the nuclear envelope, according to results from Lancaster et al. Because oocytes lack centrosomes, they assemble spindle microtubules from chromatin. By clustering chromosomes together, karyosomes help ...
متن کامل